Study of anti-tussive activity of Ocimum sanctum Linn in guinea pigs.

Aqueous and methonol ic ex t rac t o f Ocimum sanc tum were p repared f rom a i r dr ied , powdered aer ia l pa r t s o f the p lan t . The p lan t mate r ia l was ex t rac ted by ho t perco la t ions and dr ied under vacuum to remove t races o f the so lven t . The y ie lds were 18% w/w and 6.1% w/w for water and methanol ic ex t rac t s respec t ive ly . The HPTLC ana lys i s revea led the p resence of ursolic acid 1.3% in methanolic and 0.5% in aqueous extracts The chromatogram of both the ex t rac t s had s imi la r peaks by HPLC m e t h o d .